PUBLICATIONS
Peer-reviewed research, perspectives and preprints applying REAP to functional autoantibodies across human disease and therapeutic development.
REAP profiling of 174 approved or clinical-stage monoclonal antibodies found detectable off-target binding to human extracellular proteins in 28% of the panel. The study also showed that targeted engineering could remove a validated off-target interaction while preserving intended affinity and developability.
10.1016/j.str.2026.02.012 ↗
Across 352 individuals with schizophrenia and 971 controls, REAP identified a higher burden of CNS- and blood-brain-barrier-directed autoantibodies, including signals present near disease onset. Higher baseline burden was associated with poorer risperidone response; larger independent cohorts and interventional studies are needed to establish causality.
10.64898/2026.05.04.722731 ↗
REAP profiling of 374 checkpoint-immunotherapy-treated cancer patients linked specific functional autoantibodies to markedly different treatment responses. Neutralization experiments and mouse models implicated actionable pathways, including type I interferon and TL1A biology, that may modulate checkpoint efficacy.
10.1038/s41586-025-09188-4 ↗
This Perspective frames the autoantibody reactome as a layer of functional human variation extending beyond classical autoimmune disease. It argues that systematic autoantibody-wide association studies can complement genetics by identifying disease-modifying pathways and potential therapeutic targets.
10.1126/science.abn1034 ↗
The study introduced REAP, a high-throughput yeast-display platform that preserves conformational extracellular antigens for broad autoantibody discovery. Benchmarking in APS-1 and SLE showed sensitive detection of established and previously unrecognized reactivities, supporting studies across diverse diseases.
10.1016/j.crmeth.2022.100172 ↗REAP revealed broad autoantibody reactivity against cytokines, chemokines, immune-cell proteins and other extracellular targets in COVID-19, with the highest-scoring reactivities enriched in severe disease. Functional analyses showed that some autoantibodies impaired antiviral immunity and were associated with higher viral loads and altered immune-cell composition.
10.1038/s41586-021-03631-y ↗